Fibroblast Growth Factor CAS 62031-54-3

Fibroblast Growth Factor CAS 62031-54-3

Identification

CAS Number

62031-54-3

Name

Fibroblast Growth Factor

Sequence

H-Lys-Arg-Thr-Gly-Gln-Tyr-Lys-Leu-OH

Synonyms

L-Prolyl-L-alanyl-L-leucyl-L-prolyl-L-α-glutamyl-L-α-asparagylglycylglycyl-L-serylglycyl-L-alanyl-L-phenylalanyl-L-prolyl-L-prolylglycyl-L-histidyl-L-phenylalanyl-L-lysyl-L-α-asparagyl-L-p rolyl-L-lysyl-L-arginyl-L-leucyl-L-tyrosin [German] [ACD/IUPAC Name]
L-Prolyl-L-alanyl-L-leucyl-L-prolyl-L-α-glutamyl-L-α-aspartylglycylglycyl-L-serylglycyl-L-alanyl-L-phenylalanyl-L-prolyl-L-prolylglycyl-L-histidyl-L-phenylalanyl-L-lysyl-L-α-aspartyl-L-pro lyl-L-lysyl-L-arginyl-L-leucyl-L-tyrosine [ACD/IUPAC Name]
L-Prolyl-L-alanyl-L-leucyl-L-prolyl-L-α-glutamyl-L-α-aspartylglycylglycyl-L-sérylglycyl-L-alanyl-L-phénylalanyl-L-prolyl-L-prolylglycyl-L-histidyl-L-phénylalanyl-L-lysyl-L-α-aspartyl-L-pro lyl-L-lysyl-L-arginyl-L-leucyl-L-tyrosine [French] [ACD/IUPAC Name]
L-Tyrosine, L-prolyl-L-alanyl-L-leucyl-L-prolyl-L-α-glutamyl-L-α-aspartylglycylglycyl-L-serylglycyl-L-alanyl-L-phenylalanyl-L-prolyl-L-prolylglycyl-L-histidyl-L-phenylalanyl-L-lysyl-L-α-as partyl-L-prolyl-L-lysyl-L-arginyl-L-leucyl- [ACD/Index Name]
211362-85-5 [RN]
62031-54-3 [RN]
MFCD00133317

SMILES

C[C@@H](C(=O)N[C@@H](Cc1ccccc1)C(=O)N2CCC[C@H]2C(=O)N3CCC[C@H]3C(=O)NCC(=O)N[C@@H](Cc4c[nH]cn4)C(=O)N[C@@H](Cc5ccccc5)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(=O)O)C(=O)N6CCC[C@H]6C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc7ccc(cc7)O)C(=O)O)NC(=O)CNC(=O)[C@H](CO)NC(=O)CNC(=O)CNC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H]8CCCN8C(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]9CCCN9

StdInChI

InChI=1S/C118H173N31O33/c1-64(2)49-78(107(171)145-85(117(181)182)53-70-35-37-72(151)38-36-70)139-104(168)76(30-18-44-125-118(121)122)135-103(167)75(28-14-16-42-120)137-111(175)88-32-21-46-147(88)115(179)84(56-97(160)161)144-105(169)74(27-13-15-41-119)136-108(172)79(51-68-23-9-7-10-24-68)140-109(173)80(54-71-57-123-63-130-71)133-94(155)61-129-110(174)87-31-19-47-148(87)116(180)90-34-22-48-149(90)114(178)83(52-69-25-11-8-12-26-69)143-98(162)66(5)131-92(153)60-128-101(165)86(62-150)134-93(154)59-126-91(152)58-127-100(164)81(55-96(158)159)141-106(170)77(39-40-95(156)157)138-112(176)89-33-20-45-146(89)113(177)82(50-65(3)4)142-99(163)67(6)132-102(166)73-29-17-43-124-73/h7-12,23-26,35-38,57,63-67,73-90,124,150-151H,13-22,27-34,39-56,58-62,119-120H2,1-6H3,(H,123,130)(H,126,152)(H,127,164)(H,128,165)(H,129,174)(H,131,153)(H,132,166)(H,133,155)(H,134,154)(H,135,167)(H,136,172)(H,137,175)(H,138,176)(H,139,168)(H,140,173)(H,141,170)(H,142,163)(H,143,162)(H,144,169)(H,145,171)(H,156,157)(H,158,159)(H,160,161)(H,181,182)(H4,121,122,125)/t66-,67-,73-,74-,75-,76-,77-,78-,79-,80-,81-,82-,83-,84-,85-,86-,87-,88-,89-,90-/m0/s1

StdInChIKey

YSFTYXKQUONNFY-NQXPEFQPSA-N

Molecular Formula

C118H173N31O33

Molecular Weight

2553.824

MDL Number

MFCD00166724

Properties

Appearance

White or off-white lyophilized powder

Safety Data

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our Fibroblast Growth Factor CAS 62031-54-3

Description

Recombinant human basic fibroblast growth factor (also known as basic FGF, bFGF, FGF2, FGF-β or heparin-binding growth factor) is a biologically active protein suitable for cell culture applications. The bFGF regulates various processes such as cell proliferation, differentiation, survival, adhesion, exercise, apoptosis, limb formation and wound healing. The bFGF can be used for angiogenesis, fibroblast mitosis, axonal growth of PC-12 cells, receptor binding and tyrosine phosphorylation studies.
The product is lyophilized powder prepared by recombinant E.coli expression, separated and purified by multiple steps, dissolve in 10mM PBS, 0.15 M NaCl (pH7.2) solution and then sterilized by filtration through a 0.22μm filter membranes and freeze-dried.

Advantage

  1. No animal origin: no animal diseases, no pathogenic substances, no contamination by exogenous factors, and high safety.
  2. Quality stability: This product is a lyophilized powder containing a biologically active protective agent. It has been mass-produced, and the difference between batches is small; the production scale is more than 1000L.

Properties

SpeciesHuman
SourceE.coli
MW17.3KD±1.7kDa
Purity≥95%(SDS-PAGE)
Endotoxin< 0.2 EU/µg
AsepticFilter and sterilize with 0.22μm filter before freeze-drying
Isoelectric point9.5-9.6
Activity≥5.0 x 106IU /mg pro.
SolubilityGood solubility in water, buffer, and medium

Biological activity

The biological activity of the mouse fibroblast cell line BALB/3T3 was determined by the method of Chinese Pharmacopoeia 2020 edition using bFGF.

Recommended Method

 It is recommended centrifuging the powder to the bottom of the tube before opening the lid, then dissolve it in sterilized demonized water to prepare a storage solution of 100 μg/ml. It can be stored stably for 2 weeks at 2℃ ~ 8℃.When diluted to a lower concentration (not less than 10μg/ml), 0.1% tissue culture grade BSA or HSA is required. If it is necessary to extend the storage time, it is recommended to freezing at -80℃ or -20℃ rather than multigelation.This product should be established by experiment to determine the optimum concentration according to each specific application.

Transport and Storage Stability

Transportation stability: It is transported in an ice pack to keep it active. 

 Storage stability: stable to 12 months at -15~-20°C and 24 months at -80°C, stable to 4 weeks at 2~8℃. Prepare as a storage solution of 100μg/ml, stable storage at 2~8℃ for one week.

Known Application

Used in cell culture to enhance cell proliferation, cell growth and cell differentiation.

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).